LOCUS       40924_22403        4989 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGloSensor-10F[TEV], complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4989)
  AUTHORS   Wigdal SS, Frackman S, Slater M.
  TITLE     pGloSensor-10F[TEV] Control Plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4989)
  AUTHORS   Wigdal SS, Frackman S, Slater M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAY-2008) Scientific Communications, Promega 
            Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
REFERENCE   3  (bases 1 to 4989)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4989)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-MAY-2008) Scientific Communications, Promega Corporation, 2800 
            Woods Hollow Rd., Madison, WI 53711, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        23..41
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      46..63
                     /label=T7 RNA polymerase promoter
                     /note="T7 RNA polymerase promoter"
                     /regulatory_class="promoter"
     promoter        46..63
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           65..201
                     /label=BYDV 5' UTR
                     /note="barley yellow dwarf virus 5' untranslated region; 
                     required for function of the cap-independent 3' translation
                     enhancer (Guo et al., 2001)"
     CDS             1153..1173
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     misc_feature    1896..2002
                     /label=BYDV 3' TE
                     /note="cap-independent 3' translation enhancer from barley 
                     yellow dwarf virus (Wang et al., 1997)"
     terminator      2107..2154
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        2383..2487
                     /label=AmpR promoter
     CDS             2488..3345
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      complement(3506..4094)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    4210..4493
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to
                     maintain plasmids as monomers"
     terminator      4723..4809
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      4901..4928
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
ORIGIN
        1 ggatctcgat cccgcgaaat taatttaggt gacactatag aagggtaata cgactcacta
       61 tagcagtgaa gattgaccat ctcacaaaag ctgttacgtg cttgtaacac actacacact
      121 cgttttgtat tcgagaagta gttgcaacaa cggtcccctt attgcctgac aagctgaggg
      181 ccacccttct atccccaccg cgcgatcgcc atggacaccg ctatcctcag cgtggtgcca
      241 tttcaccacg gcttcggcat gttcaccacg ctgggctact tgatctgcgg ctttcgggtc
      301 gtgctcatgt accgcttcga ggaggagcta ttcttgcgca gcttgcaaga ctataagatt
      361 caatctgccc tgctggtgcc cacactattt agcttcttcg ctaagagcac tctcatcgac
      421 aagtacgacc taagcaactt gcacgagatc gccagcggcg gggcgccgct cagcaaggag
      481 gtaggtgagg ccgtggccaa acgcttccac ctaccaggca tccgccaggg ctacggcctg
      541 acagaaacaa ccagcgccat tctgatcacc cccgaagggg acgacaagcc tggcgcagta
      601 ggcaaggtgg tgcccttctt cgaggctaag gtggtggact tggacactgg taagacactg
      661 ggtgtgaacc agcgcggcga gctgtgcgtc cgtggcccca tgatcatgag cggctacgtt
      721 aacaaccccg aggctacaaa cgctctcatc gacaaggacg gctggctgca cagcggcgac
      781 atcgcctact gggacgagga cgagcacttc ttcatcgtgg accggctgaa gagcctgatc
      841 aaatacaagg gctaccaggt agccccagcc gaactggaga gcatcctgct gcaacacccc
      901 aacatcttcg acgccggggt cgccggcctg cccgacgacg atgccggcga gctgcccgcc
      961 gcagtcgtcg tgctggaaca cggtaaaacc atgaccgaga aggagatcgt ggactatgtg
     1021 gccagccagg ttacaaccgc caagaagctg cgcggtggtg ttgtgttcgt ggacgaggtg
     1081 cctaaaggac tgaccggcaa gttggacgcc cgcaagatcc gcgagattct cattaaggcc
     1141 aagaaggcta gcgagaacct gtacttccag agcggatctg ccaaaaacat taagaagggc
     1201 ccagcgccat tctacccact cgaagacggg accgccggcg agcagctgca caaagccatg
     1261 aagcgctacg ccctggtgcc cggcaccatc gcctttaccg acgcacatat cgaggtggac
     1321 attacctacg ccgagtactt cgagatgagc gttcggctgg cagaagctat gaagcgctat
     1381 gggctgaata caaaccatcg gatcgtggtg tgcagcgaga atagcttgca gttcttcatg
     1441 cccgtgttgg gtgccctgtt catcggtgtg gctgtggccc cagctaacga catctacaac
     1501 gagcgcgagc tgctgaacag catgggcatc agccagccca ccgtcgtatt cgtgagcaag
     1561 aaagggctgc aaaagatcct caacgtgcaa aagaagctac cgatcataca aaagatcatc
     1621 atcatggata gcaagaccga ctaccagggc ttccaaagca tgtacacctt cgtgacttcc
     1681 catttgccac ccggcttcaa cgagtacgac ttcgtgcccg agagcttcga ccgggacaaa
     1741 accatcgccc tgatcatgaa cagtagtggc agtaccggat tgcccaaggg cgtagcccta
     1801 ccgcaccgca ccgcttgtgt ccgattcagt catgcccgcg accccatctt cggcaaccag
     1861 atcatccccg tttaaacgaa ttcgagctcg gtaccagtga agacaacacc actagcacaa
     1921 atcggatcct gggaaacagg cagaacttcg gttcataagc tcgggtaggc tgtcaaccta
     1981 ccgccgtatc gtattgtgtt tgtctagata cgtatcgcga gtcgacctgc aggcatgcaa
     2041 gctgatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag
     2101 caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa
     2161 ggaggaacta tatccggttc gcttgctgtc cataaaaccg cccagtctag ctatcgccat
     2221 gtaagcccac tgcaagctac ctgctttctc tttgcgcttg cgttttccct tgtccagata
     2281 gcccagtagc tgacattcat ccggggtcag caccgtttct gcggactggc tttctacgta
     2341 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     2401 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     2461 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     2521 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     2581 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     2641 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cactttcaaa
     2701 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     2761 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     2821 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     2881 gcggccaact tacttctgac aactatcgga ggaccgaagg agctaaccgc ttttttgcac
     2941 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     3001 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     3061 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     3121 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     3181 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     3241 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     3301 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaatt cgaaatgacc
     3361 gaccaagcga cgcccaaccg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     3421 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     3481 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     3541 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     3601 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     3661 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     3721 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     3781 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     3841 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     3901 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     3961 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     4021 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     4081 aaaaaggatt tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     4141 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     4201 ttttatagtc cggaaataca ggaacgcacg ctggatggcc cttcgctggg atggtgaaac
     4261 catgaaaaat ggcagcttca gtggattaag tgggggtaat gtggcctgta ccctctggtt
     4321 gcataggtat tcatacggtt aaaatttatc aggcgcgatt gcggcagttt ttcgggtggt
     4381 ttgttgccat ttttacctgt ctgctgccgt gatcgcgctg aacgcgtttt agcggtgcgt
     4441 acaattaagg gattatggta aatccactta ctgtctgccc tcgtagccat cgagataaac
     4501 cgcagtactc cggccacgat gcgtccggcg tagaggatcg ggatcttttc agcctgatac
     4561 agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc ggcagtagcg
     4621 cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta
     4681 gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct
     4741 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
     4801 aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg
     4861 gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat cctgacggat
     4921 ggcctttttg cgtttctaca aactcttttg tttatttttc taaatacatt caaatatgta
     4981 tccgctcat
//
